elisa standard curve fit

[trevor g]

I am a biochemist and I am wanting to be able to plot immunoassay (ELISA)
data. In particular I want to be able to produce standard curves using a
4-parameter logistic curve fit and read the values of 'unknown' samples from
the curve.

 

[Bernard Liengme]

I can show you how to fit a logistics curve using Solver - Write to my
private email - I
Also try Google with <ELISA curve> and with <ELISA logistic Excel>
best wishes

 

[assay wiz]

Try http://www.myassays.com/four-parameter-fit.assay

This has everything you need to do a 4PL with standards and unknowns and it's free. Also, there is an Add-in for Excel available at http://www.myassays.com/introducing-excel-add-in.html

 

[Charles Ma]

Hi Trevor,

I know this a "bit" late as a reply. But I hope this can help you and others who landed here. We have a FREE, easy to use online tool for ELISA analysis at www.readerfit.com. It is able to fit your standard curve with 4-parameter logistic and 5-parameter logistic as well. If you come to our site, wehave great email/live support where we can walk you through a typical analysis.

Charles Ma
Technical Support
Hitachi Solutions America, Ltd.
MiraiBio Group
Website: http://www.MiraiBio.com

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Check out our new online free ELISA analysis tool at http://www.ReaderFit.com
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[ericvandamme18]

Hi Trevor,

I think IVDfit may be something for you. IVDfit is an Excel add-in. It can do 4PL and 5PL and it is specially made for ELISA data. You can also calculate your unknowns with it. It can be downloaded at www.ivdtools.com